This module provides a theoretical introduction and direct practical training in several techniques used in eukaryote cell biology. Students will learn about the holistic aspects of cell culture, gain a solid understanding of the use of various cell culture techniques and be able to appreciate their application in different fields of research and healthcare. Students will also have hands-on experience in the basic techniques of in-vitro mammalian cell culture, proliferation, fluorescence microscopy, cryopreservation, gene expression through transfection procedures, and isolation of primary cell cultures. After going through the module, students will appreciate how different techniques can be used to study the biology of the cell, and how they can be used in combination to answer specific questions relating to research of cell functions and their extended use in the pharmaceutical industry.